|Alumina for Column Chromatoraphy||1344-28-1|
|Silica Gel for Column Chromatography||112926-00-8|
|Silica Gel for TLC||112926-00-8|
Acme Synthetic Chemicals is the Manufacturer, Supplier and also Exporter of Silica Gel and Aluminium Oxide Chromatography Adsorbents.
We offer Aluminium Oxide for column chromatography (also called Activated Alumina) in three types based on the pH ranges – Acidic Alumina , Neutral Alumina, and Basic Alumina.
We offer Silica Gel in various mesh sizes such as 30-70, 60-120, 70-230, 100-200, 200-300, and 230-400.
Acme’s Silica Gel is produced in fully enclosed environments under controlled conditions unlike many other brands which are manufactured in open air and contain extraneous impurities.
WHAT IS CHROMATOGRAPHY?
Chromatography is the term used for a variety of laboratory techniques that are used for the separation of different constituents of a mixture.
It is a physical method of separation not involving any chemical reactions.
In the method, the mixture is dissolved in a fluid which is called the mobile phase, which carries it through another material called the stationary phase.
Separation is due to the different rates of passage through the stationary phase resulting in differential retention on the stationary phase.
The different rates of passage are due to the interaction of the constituents in the mixture with the stationary phase and this interaction depends on the affinity of the constituents with the stationary phase material.
There are several chromatographic methods of which two very popular chromatographic separation techniques are Column Chromatography and Thin Layer Chromatography.
Column Chromatography is done by dissolving the mixture into a solvent or a mixture of solvents called the eluent.
The mixture is then loaded into a column packed with finely powdered adsorbent which is usually Aluminium Oxide (Alumina) or Silica Gel
Compounds with different polarity partition to different extents and therefore move through the column at different rates.
The individual compounds are collected as successive eluent fractions.
Thin Layer Chromatography (TLC)
Thin Layer chromatography is performed on a sheet of glass, plastic, or aluminium, which is coated with an inert thin layer of adsorbent material, usually Silica Gel or Aluminium Oxide (Alumina).
Spots of the mixture are applied to the plate using very thin glass pipettes.
The plate is then placed in a glass jar containing the solvent or solvent mixture which is then drawn up the plate via capillary action.
Because different analytes ascend the TLC plate at different rates, separation is achieved.